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|Title:||Bacterial molecular characterization in intraoral appliances: Its role in oral pathogenicity and immunity [poster]||Autores:||Féliz-Matos, Leandro
Hernández, Luis Miguel
|Researchers (UNIBE):||Féliz-Matos, Leandro
Hernández, Luis Miguel
|Affiliations:||Centro de Investigación en Biomateriales y Odontología (CIBO)
Centro de Investigación en Biomateriales y Odontología (CIBO)
Instituto de Medicina Tropical y Salud Global (IMTSAG)
|Research area:||Ciencias de la Salud||Issue Date:||2016||Source:||International Dental Journal, 66(S1)||Journal:||International Dental Journal||metadata.dc.identifier.artno:||FC145||Volume:||66||Issue:||S1||Abstract:||
Aim or purpose: To characterize bacterial growth in intraoral appliances and describe its pathogenicity and Immunity.
Materials and methods: Seventeen male and 35 females (18–65 years) were recruited from UNIBE’s Dental Clinic. The proto-col was approved by UNIBE’s IRB. Oral swabs were used to collect the samples from different intraoral appliances, such as orthodontic and prosthetic (ﬁxed and removable), and its contacting mucosa. Collected samples were cultured in speciﬁc growth culture media. Culture for fastidious aerobic and anaerobic Gram+ and Gram microorganisms were also used. DNA extractions and restriction enzyme were applied with standardized methods to the isolates.
Results: Identiﬁcation of Porphyromonas gingivalis, Prevotella intermedia, Bacteroides fragilis, and Actinomyces israelli was detected in the intraoral appliances. P. gingivalis and A. israelii were more frequent in both sexes.
Conclusion: It is unlikely to ﬁnd P. gingivalis in healthy environments, interacting with the host immunity, and inducing a pro-inﬂammatory response that manipulates host-to-microbe responses. P. intermedia, and B. fragilis are bioﬁlm related bacteria. However, B. fragilis can harm the colonic epithelial tissue and develop intestinal inﬂammation. A. israelli is normally found in the intraoral, gastrointestinal, genital, and oropharyngeal ﬂora and is related to the etiology of actynomicosis. The frequent pathologies associated to A. israelli are cervicofacial fungal infections, abdominal pain, colon ulcerations and disorders of central nervous system; the presence of bioﬁlm in intraoral appliances can be a predisposing factor. This bacterium seems to interact with host responses; more studies exploring interactions with autoimmune or inﬂammatory markers in humans are need.
|Appears in Collections:||Publicaciones del CIBO-UNIBE|
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